Reagents and instruments for immunology, cell biology and molecular biology.

Anti-SOX10 antibody (350-450) (Clone: S6MR)

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Cat# NB-22-48765-200

Size : 200uL(2x100uL)

Brand : Neo Biotech

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Western blot analysis of extracts of various cell lines, using SOX10 rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunoprecipitation analysis of 300 Mu g extracts of C6 cells using 3 Mu g SOX10 antibody . Western blot was performed from the immunoprecipitate using SOX10 antibody at a dilution of 1:1000.
Immunofluorescence analysis of C6 cells using SOX10 rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Confocal imaging of C6 cells using SOX10 Rabbit monoclonal antibody followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunoprecipitation analysis of 300ug extracts of C6 cells using 3ug SOX10 antibody . Western blot was performed from the immunoprecipitate using SOX10 antibody at a dilition of 1:1000.
Immunohistochemistry analysis of SOX10 in paraffin-embedded rat brain tissue using SOX10 Rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of SOX10 in paraffin-embedded human breast tissue using SOX10 Rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of SOX10 in paraffin-embedded human brain tissue using SOX10 Rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of SOX10 in paraffin-embedded mouse brain tissue using SOX10 Rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Western blot analysis of various lysates using SOX10 Rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

General Info

Host:	Rabbit
Applications:	WB/IF/IP
Reactivity:	Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-SOX10 (350-450) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.

Clonality:	Monoclonal
Clone ID:	S6MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IF/ICC 1:50-1:200 IP 1:500-1:1000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	SOX10
Gene ID:	6663
Uniprot ID:	SOX10_HUMAN
Immunogen Region:	350-450
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human SOX10 (P56693).
Immunogen Sequence:	VDAKAQVKTETAGPQGPPHY TDQPSTSQIAYTSLSLPHYG SAFPSISRPQFDYSDHQPSG PYYGHSGQASGLYSAFSYMG PSQRPLYTAISDPSPSGPQS H

Description

Tissue Specificity	Expressed in fetal brain and in adult brain, heart, small intestine and colon.
Function	Transcription factor that plays a central role in developing and mature glia. Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Once induced, MYRF cooperates with SOX10 to implement the myelination program. Transcriptional activator of MITF, acting synergistically with PAX3. Transcriptional activator of MBP, via binding to the gene promoter.
Protein Name	Transcription Factor Sox-10
Database Links	Reactome: R-HSA-9619665 Reactome: R-HSA-9764302
Cellular Localisation	Cytoplasm Nucleus Mitochondrion Outer Membrane Peripheral Membrane Protein Cytoplasmic Side
Alternative Antibody Names	Anti-Transcription Factor Sox-10 antibody Anti-SOX10 antibody

Information sourced from Uniprot.org

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