Reagents and instruments for immunology, cell biology and molecular biology.

Anti-ICAM1 antibody (1-100) (Clone: S3MR)

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Cat# NB-22-48092-200

Size : 200uL(2x100uL)

Brand : Neo Biotech

Western blot analysis of extracts of A-549 cells, using ICAM-1/CD54 antibody at 1:1000 dilution. A-549 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using ICAM-1/CD54 antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Flow cytometry:1X10^6 293F cells (negative control, left) and Raji cells (right) were surface-stained with ICAM-1/CD54 rabbit monoclonal antibody or rabbit IgG isotype control (10 Mu g/mL, blue line) , followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

General Info

Host:	Rabbit
Applications:	WB/IHC/FC
Reactivity:	Human
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-ICAM1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry research applications.

Clonality:	Monoclonal
Clone ID:	S3MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IHC-P 1:50-1:200 FC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	ICAM1
Gene ID:	3383
Uniprot ID:	ICAM1_HUMAN
Immunogen Region:	1-100
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ICAM-1/CD54 (P05362).
Immunogen Sequence:	MAPSSPRPALPALLVLLGAL FPGPGNAQTSVSPSKVILPR GGSVLVTCSTSCDQPKLLGI ETPLPKKELLLPGNNRKVYE LSNVQEDSQPMCYSNCPDGQ

Description

Post Translational Modifications	Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Function	ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
Protein Name	Intercellular Adhesion Molecule 1 Icam-1 Major Group Rhinovirus Receptor Cd Antigen Cd54
Database Links	Reactome: R-HSA-198933 Reactome: R-HSA-216083 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-877300
Cellular Localisation	Membrane Single-Pass Type I Membrane Protein
Alternative Antibody Names	Anti-Intercellular Adhesion Molecule 1 antibody Anti-Icam-1 antibody Anti-Major Group Rhinovirus Receptor antibody Anti-Cd Antigen Cd54 antibody Anti-ICAM1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance