Reagents and instruments for immunology, cell biology and molecular biology.
 
> Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

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Cat# E-BC-K019-M-96T

Size : 96T

Brand : Elabscience

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Detection Principle The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.
Detection Principle Image
Synonyms T-SOD
Sample Type Serum,Plasma,Urine,Cell,Cell culture supernatant,Tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (540-560 nm, optimum wavelength: 550 nm)
Research Area Oxidative Stress ,  Plant Physiology Series
Other Reagents Required PBS (0.01 mol/L, pH 7.4), Normal saline (0.9% NaCl)
Storage This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period 12 months
Sensitivity 2.4 U/mL
Detection Range 2.4-61 U/mL
Precision inter-assay CV: 5.6% | intra-assay CV: 5.5%
Assay Time 1 h 10 min
Cat.No. Product Name Sizes
E-BC-K020-M Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method) 500Assays , 96T , 48T
E-BC-K022-M CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method) 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 2-4
Rat serum 4-6
Mouse serum 4-6
HepG2 cell 15-30
Human urine 2-5
10% Mouse liver tissue homogenate 160-200
10% Epipremnum aureumtissue homogenate 3-5
10% Mouse brain tissue homogenate 80-100

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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