Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit
Detection principle
Performance characteristics
Synonyms | T-GSH,GSSG |
Sample type | Serum,plasma,animal tissue,red blood cells,cultured cells |
Sensitivity | 0.12 μmol/L T-GSH |
Detection range | 0.12-30 μmol/L T-GSH |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 180 min |
Precision | Average inter-assay CV: 4.700%Average intra-assay CV: 0.900% |
Other instruments required | Micropipettor, Incubator, Vortex mixer, Centrifuge |
Other reagents required | PBS(0.01 M, pH 7.4), Absolute ethanol |
Storage | Reagent 2, Reagent 3, Reagent 4, Reagent 8, Reagent 9: -20℃; others: 2-8℃ |
Valid period | 12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-30 μmol/L T-GSH).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type | Dilution factor |
Human serum | 1 |
Rat plasma | 1 |
Mouse serum | 1 |
10% Rat heart tissue homogenate | 10 |
10% Rat liver tissue homogenate | 60 |
10% Rat brain tissue homogenate | 10 |
Note: The diluent is reagent 3.
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits
Citations
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Bioengineered (2022) IF: 3.269
SNAI2 promotes the development of ovarian cancer through regulating ferroptosisPMID: 35220872
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PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathwayPMID: 35635784
Sample: Brain Tissue -
Porcine Health Management (2022) IF: 3.535
Is a new generation of mycotoxin clay adsorbents safe in a pig’s diet?Sample: Blood -
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2021) IF: 3.639
Effects of almonds on ameliorating salt-induced hypertension in Dahl salt-sensitive ratsDOI: 10.1002/jsfa.11611
PMID: 34708426
Sample: Renal Cortex -
Food & Function (2021) IF: 5.396
Supplementation of amino acids and organic acids prevents the increase in blood pressure induced by high salt in Dahl salt-sensitive ratsDOI: 10.1039/D1FO03577K
PMID: 34994761
Sample: Renal -
ENVIRONMENTAL MICROBIOLOGY (2021) IF: 5.491
A novel transcription factor negatively regulates antioxidant response, cell wall integrity and virulence in the fungal insect pathogen, Beauveria bassianaPMID: 33432709
Sample: Cell Sample -
FOOD CHEMISTRY (2021) IF: 7.514
Cytotoxicity of adducts formed between quercetin and methylglyoxal in PC-12 cellsPMID: 33706136
Sample: Cell Lysate