Reagents and instruments for immunology, cell biology and molecular biology.

Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)

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Cat# E-BC-K109-M-48T

Size : 48T

Brand : Elabscience

Detection Principle	Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of 4-aminoamylpyridine and phenol, hydrogen peroxide catalyze peroxidase to form red quinone compounds of benzoquinone imine phenizone.The color depth of the generated quinone is directly proportional to the cholesterol content. The absorbance values of the standard tube and the sample tube are measured respectively, and the cholesterol content in the sample can be calculated.
Synonyms	TC
Sample Type	Serum,Plasma,Tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (495-525 nm)
Research Area	Metabolic Diseases , Glycolysis And Lipid Metabolism
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Anhydrous ethanol
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.29 mmol/L
Detection Range	0.29-25.85 mmol/L
Precision	inter-assay CV: 8.3% \| intra-assay CV: 3.1%
Assay Time	20 min

Cat.No.	Product Name	Sizes
E-BC-K261-M	Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)	500Assays , 96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Mouse serum	1
Rat plasma	1
10% Mouse liver tissue homogenate	1
10% Mouse kidney tissue homogenate	1
10% Mouse heart tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.