Reagents and instruments for immunology, cell biology and molecular biology.

TLR4 (Toll Like Receptor 4, CD284) (APC)

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Cat# T8050-34-APC-100ul

Size : 100ul

Brand : US Biological

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T8050-34-APC TLR4 (Toll Like Receptor 4, CD284) (APC)

Clone Type

Polyclonal

Host

mouse

Source

human

Isotype

IgG2a

Grade

Affinity Purified

Applications

Crossreactivity

Bo Ca Gp Hu Mk Po

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.|TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. This antibody has been demonstrated to block activation of monocytes with LPS. Removal of sodium azide is recommended prior to use in functional assays. ||Applications:|Suitable for use in Immunoprecipitation. Other applications not tested.||Recommended Dilution:|Optimal working dilutions to be determined by researcher.||Hybridoma: |Sp2/0 myeloma cells with spleen cells from Balb/c mice. ||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG2a|Clone No: 3F5 (HTA125)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Ba/F3 cell line expressing TLR4|Specificity: Recognizes human TLR4 (Toll Like Receptor 4). Has been shown to block the activation of monocytes with LPS. Species Crossreactivity: canine, rhesus monkey, guinea pig, porcine, bovine and mouse||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Ba/F3 cell line expressing TLR4

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Purity

Purified by Protein A affinity chromatography from tissue culture supernatant.

Specificity

Recognizes human TLR4 (Toll Like Receptor 4). Has been shown to block the activation of monocytes with LPS. Species Crossreactivity: canine, rhesus monkey, guinea pig, porcine and bovine

References

1. Shimazu, R., et al., J. Exp. Med. 189: 1,777-1,782 (1999). 2. Jiang, Q., et al., J. Immunol. 165: 3,541-3,544 (2000). 3. Yang, S., et al., Infect. Immun. 69: 2,045-2,053 (2001). 4. Triantafilou, M., et al., J. Cell Sci. 115: 2,603-2,611 (2002).