Orb1821442-10ml | SYBR Green Fluorescent DNA Stain Biotrend USA

SYBR Green Fluorescent DNA Stain

Catalog Number	orb1821442
Category	Tools
Description	SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. SYBR® Green Fluorescent DNA Stain is supplied as 100 µM con-centration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 µM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors. Select the optical setting for SYBR® Green or FAM on the detection instrument. final SYBR® Green concentration20 µl PCR assay 50 µl PCR assay 0.5 µM 0.1 µl 0.25 µl 1.0 µM 0.2 µl 0.50 µl SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.
Concentration	100 µM
Form/Appearance	liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20; Color: orange
Storage	store at -20 °C. store dark
Note	For research use only
Application notes	< b>Spectroscopic Propertie: λexc 495 nm, λem 520 nm (bound to DNA).
Expiration Date	12 months from date of receipt.