SUMO Protease
Cat# K1103-5000
Size : 5000U
Brand : APExBIO Technology
SUMO Protease
SUMO protease, also known as Ulp, is a highly active cysteine protease derived from a recombinant gene fragment of Ubl-specific protease 1 in Saccharomyces cerevisiae. SUMO proteases recognize and cleave peptide bonds after the SUMO carboxyl (C-terminal) of the Ubiquitin-like (UBL) protein in a highly specific manner. SUMO is an Ubiquitin-like protein that is commonly found in post-translation modification (PTM), which plays an important role in the stability of proteins and the regulation of biological functions.
The optimal lysis temperature for SUMO proteases is 30 , but it is in the wide pH range (6.0-10.0) and temperature range (2 to 30 -400mM NaCl) maintains high enzyme activity. In special scenario use, the sample can be digested overnight at 4~2mM), and the addition of DTT at the appropriate concentration to the digestion system can significantly improve the digestion efficiency, especially during long-term digestion, such as Enzymes are cut overnight at 4 >Quality Control
| Components | Detailed composition | Specification | ||
| 200U | 1000U | 5000U | ||
| SUMO Protease (10U/ mM Tris-HCl, pH 8.0 0.1% Igepal (NP-40) 250 mM NaCl 500 μM DTT 50% (v/v) glycerol | 20 X SUMO Protease Buffer + Salt | 500 mM Tris acetate, pH 8.0 2% Igepal (NP-40) 1.5 M NaCl 10 mM DTT | 400 ×1 ml | 10×1 ml |
| 10X SUMO Protease Buffer mM Tris acetate, pH 8.0 2% Igepal (NP-40) 10 mM DTT | 400 ×1 ml | 10×1 ml | ||
Long-term storage: -80 ).children().addClass('active'); " + "px"}, 500); }); }); ); var items = calItems > 8?8:calItems; owl.owlCarousel({ items: items, autoPlay: 3000, dots: false, pagination:false, navigation:true, responsive:{ 0 : { items:2, }, 480 : { items:4, }, 768 : { items:6, }, 1200:{ items:8, } } }); }); | ||||