ROS Detection Assay Kit (DCFDA / H2DCFDA)

Cat# CA093

Size : 5×96assays

Brand : Canvax Biotech

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ROS Detection Assay Kit (DCFDA / H2DCFDA)

Highly Sensitive & Safe Assay for Reactive Oxygen Species Measurement

ROS Detection AssayO2), hydroxyl radical (HOO2), all of which are more reactive than oxygen (O2) itself. The molecules are produced during the electron transport of mitochondrial aerobic respiration or by oxidoreductase enzymes and metal catalysed oxidation.

It uses the cell permeant reagent Dichlorodihydrofluorescein-diacetate (H2DCFDA), a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, the acetyl groups on H2DCFDA are cleaved by intracellular esterase to yield the non-fluorescent compound which is rapidly oxidized to highly fluorescent 2_tb_body_wrapper et_pb_module "> 5 × 96 assays

SKU: CA093 Categories: Cell Based Assays, Stress Oxidative

Detailed information:

  • Based on unique fluorescent probe H2DCFDA.
  • Fast & Easy Protocol.
  • Safe: non-Radioactive, no special handling_tb_body et_pb_toggle_close">

– 5 mgDCFDA)
– mLX Assay Buffer
– μLO2, 8.8M)
– 1 mL_tb_body et_pb_toggle_close">

Datasheet
MSDS

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  • Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
  • Shipment _tb_body et_pb_toggle_close">
    • Martínez Cid, P. (2024). Innovación en modelos in vitro: Resistencia a la ferroptosis en células de cáncer de ovario epitelial.
    • López Calvo, I. (2023). Estudio del papel de la ferroptosis en la resistencia al tratamiento quimioterapéutico en Cáncer de Ovario.
    • Parn, K. W. (2023)./">Safety and efficacy of oral epigallocatechin gallate in attenuating hypertension in spontaneously hypertensive rats via modulation of the intrarenal renin-angiotensin system; & Fernandez, R. (2022). Genomic exaptation and convergent evolution paved the way to independent subterranean colonization across beetle lineages.
    • Luparello, C., Branni, R., Abruscato, G., Lazzara, V., Drahos, L., Arizza, V., … & Vazzana, M. (2022). Cytotoxic capability and the associated proteomic profile of cell-free coelomic fluid extracts from the edible sea cucumber Holothuria tubulosa on HepG2 liver cancer cells., 722-743.
    • López-Grueso, M. J., Lagal, D. J., García-Jiménez, Á. F., Tarradas, R. M., Carmona-Hidalgo, B., Peinado, J., … & Padilla, C. A. (2020). Knockout of PRDX6 induces mitochondrial dysfunction and cell cycle arrest at G2/M in HepG2 hepatocarcinoma cells..
    • Criel, H. (2019).">INTRACELLULAR ACCUMULATION OF QUERCETIN AND ITS IMPACT ON CELLULAR STRESS: AN IN VITRO STUDY). Cytotoxic Activity of the Histone Deacetylase 3-Selective Inhibitor Pojamide on MDA-MB-231 Triple-Negative Breast Cancer Cells.(4), 804.

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