Reagents and instruments for immunology, cell biology and molecular biology.
 
> Rat N-terminal procollagen III propeptide (PIIINP) ELISA Kit

Rat N-terminal procollagen III propeptide (PIIINP) ELISA Kit

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Cat# KTE100502-96

Size : 96T

Brand : Abbkine

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Specification

Product name Rat N-terminal procollagen III propeptide (PIIINP) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat N-terminal procollagen III propeptide (PIIINP) ELISA Kit employs a two-site sandwich ELISA to quantitate PIIINP in samples. An antibody specific for PIIINP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIIINP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIIINP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIIINP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PIIINP

Product Properties

Kit components minutes before opening.
minutes.
- 8 class="pro-form-title">Additional Information
Background Plasma levels of PIIINP a marker of extracellular matrix metabolism activity have been linked to abdominal aortic aneurysm growth rate, but its relationship with thoracic aortic aneurysm (TAA) growth has never previously been studied. The PIIINP molecule is an indicator of both the synthesis and the degradation of type III collagen. Occasionally, the removal of PIIINP from the newly-synthesized type III collagen is not complete and the propeptide may remain attached to some of the molecules, known as type IIIpN-collagen. PIIINP is a degradation product of type IIIpN collagen. PIIINP has a molecular weight of 42 000 and contains three distinct domains: a triple-helical domain (Col 3) in the middle of the molecule, the Col 1-domain at the aminoterminal and Col 2-domain at the carboxyterminal end of the propeptide. PIIINP is cleared from the circulation by scavenger receptors in liver endothelial cells.
Alternative PIIINP

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