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Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western blot analysis of HepG2 cell lysate with RAN polyclonal antibody (Cat # PAB19796) at 1:200 dilution.

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of RAN.

    Immunogen

    A synthetic peptide corresponding to 14 amino acids at N-terminus of human RAN.

    Host

    Rabbit

    Reactivity

    Chicken, Dog, Human, Mouse, Rat, Xenopus

    Form

    Liquid

    Purification

    Protein A purification

    Recommend Usage

    ELISA (1:10000-40000)
    Western Blot (1:200-500)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In serum (0.02% sodium azide)

    Storage Instruction

    Store at 4°C for three months. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western blot analysis of HepG2 cell lysate with RAN polyclonal antibody (Cat # PAB19796) at 1:200 dilution.

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — RAN

    Entrez GeneID

    5901

    Protein Accession#

    P17080

    Gene Name

    RAN

    Gene Alias

    ARA24, Gsp1, TC4

    Gene Description

    RAN, member RAS oncogene family

    Omim ID

    601179

    Gene Ontology

    Hyperlink

    Gene Summary

    RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq

    Other Designations

    OK/SW-cl.81|RanGTPase|guanosine triphosphatase Ran|member RAS oncogene family|ras-related nuclear protein

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