qPCR & Sequencing Chromatrap Native Pro-A ChIP kit for enzymatic shearing only
Cat# 500237
Size : 24Units
Brand : Chromatrap
Perfect for qPCR and next-generation sequencing
Chromatrap® Native ChIP kit provides an alternative to traditional cross-linked ChIP, which removes the need for fixing cells prior to extraction. Performing ChIP with native chromatin enables users to gain insights to histone and certain abundant transcription factors that are likely to be naturally bound to DNA.
This kit is designed to enable researchers to efficiently enrich chromatin and detect binding of low abundant transcription factors and histone modifications with superior selectivity and specificity from the smallest quantity of starting material. Since this unique filter-based kit contains users benefit from the inherent low background of a ChIP technology that performs faster and is dramatically easier to use than conventional kits.
- Maximum capture of the antibody-chromatin complex
- Spin column format for maximum recovery of the enriched DNA
- Quick and easy steps from start-to-finish
- All reagents for 24 ChIP and 10 chromatin extractions supplied
- High quality NGS-ready ChIP DNA
Correctly orientated proteins for maximum chromatin-antibody binding and capture
ChIP from as little as 1000 cells
No lengthy blocking or overnight steps
FASTQ > 30
Greater than 95% uniquely mapped reads for transcription factors and histone modifications
All reagents supplied to perform 24 bead-free Protein A, ChIP assays and up to 10 chromatin sample preparations by enzymatic digestion. DNA purification components included to obtain high quality DNA for NGS library preparation.
All reagents supplied to perform 24 bead-free Protein G, ChIP assays and up to 10 chromatin sample preparations by enzymatic digestion. DNA purification components included to obtain high quality DNA for NGS library preparation.