PSMB9 monoclonal antibody, clone AEFO-16
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- More Files
Western Blot (Cell lysate)
Western blot analysis of A-431 cell lysate.
- Specifications
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human PSMB9.
Immunogen
A synthetic peptide corresponding to human PSMB9.
Host
Rabbit
Reactivity
Human
Specificity
The antibody reacts with human PSMB9, in native form and recombinant. Superfamily members of PSMB9 are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Cell lysate)
Western blot analysis of A-431 cell lysate.Immunocytochemistry
Immunofluorescence
Flow Cytometry
- Gene Info — PSMB9
Entrez GeneID
5698Protein Accession#
P28065Gene Name
PSMB9
Gene Alias
LMP2, MGC70470, PSMB6i, RING12, beta1i
Gene Description
proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
Omim ID
177045Gene Ontology
HyperlinkGene Summary
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding different isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq
Other Designations
OTTHUMP00000029423|OTTHUMP00000062982|low molecular mass protein 2|macropain chain 7|multicatalytic endopeptidase complex chain 7|proteasome beta 9 subunit|proteasome catalytic subunit 1i|proteasome chain 7|proteasome subunit beta 6i|proteasome-related ge
- Interactomes
- Pathways
- Diseases