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> Polyphenol Oxidase (PPO) Activity Assay Kit

Polyphenol Oxidase (PPO) Activity Assay Kit

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Cat# E-BC-K259-M-96T

Size : 96T

Brand : Elabscience


Detection principle

Polyphenol oxidase (PPO) can catalyze phenolic compounds into quinone substances. The latter has specific absorption at 410 nm. The activity of PPO can be calculated indirectly bymeasuring the OD value at 410 nm.

Performance characteristics

Synonyms PPO
Sample type Plant tissue
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 25 min
Precision Average inter-assay CV: 8.700%Average intra-assay CV: 4.600%
Other instruments required Tubes, Micropipette, Vortex mixer, 100℃ Water bath
Storage 2-8℃
Valid period 12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

  1. LWT-FOOD SCIENCE AND TECHNOLOGY (2021) IF: 4.952
    Bio-electrochemical impedance analysis of frozen Japanese pear tissues: And the relationships among the physical properties, total polyphenol content, and oxidase activity

    DOI: 10.1016/j.lwt.2021.112499
    Sample: Fruit
  2. Frontiers in Plant Science (2022) IF: 5.754
    MC03g0810, an Important Candidate Gene Controlling Black Seed Coat Color in Bitter Gourd (Momordica spp.).

    DOI: 10.3389/fpls.2022.875631
    Sample: Seed Coat

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