Pepsin porcine ca. 15 milliAnson units/mg
Cat# 31820.01
Size : 1G
Brand : SERVA Electrophoresis
(Pepsin A)
EC 3.4.23.1 • Mr ca. 36 000 • CAS [9001-75-6]
EC 3.4.23.1 • Mr ca. 36 000 • CAS [9001-75-6]
For the specific digestion of proteins prior to mass spectrometric analyses and further applications in protein research. Endoprotease, cleaves carboxyterminally at the position of the aromatic amino acids phenylalanine, tyrosine and tryptophan. Can also be used in combination with other proteases.
Unit definition: 1 milliAnson unit is equivalent to 1 µmole of Folin-positive amino acids calculated as tyrosine, released from denatured hemoglobin per minute at 37 °C, pH 2.0.
Activity in other units: ca. 0.015 PUHb/mg ("Pepsin-Unit" according to Anson (1, 2). Expressed in millimole tyrosine, therefore 1 PUHb = 1000 milliAnson units.)
References:
- Anson, M.L. (1938) J. Gen. Physiol. 22, 79-98
- Ryle, A.P. (1984) Methods of Enzymatic Analysis, (Bergmeyer, H.U. ed.) 3rd Ed. vol. 5, 223-38
- Northrop, J.H. et al. (1948) Crystalline Enzymes 2nd ed., Columbia University Press, 305-8
DANGER |
Hazard Statements | H315 - H319 - H334 - H335 |
Precautions | P261 - P280 |
Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 |
EG-Index: 647-008-00-6 • EINECS: 232-629-3 • WGK: 1 • HS: 35079090
Storage Temperature: -15 °C to -25 °C