Mouse Reactive oxygen species (ROS) ELISA Kit

Cat# KTE71621-5x96

Size : 96Tx5

Brand : Abbkine

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Specification

Product name Mouse Reactive oxygen species (ROS) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes Mouse Reactive oxygen species (ROS) ELISA Kit employs a two-site sandwich ELISA to quantitate ROS in samples. An antibody specific for ROS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ROS present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Mouse ROS detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of ROS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA

Product Properties

Calibration range 30 IU/mL-480 IU/mL
Limit of detection 1.0 IU/mL
Usage notes minutes before opening.
minutes.
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