Mouse Nesprin-2 (SYNE2) ELISA Kit
Cat# KTE70287-50x96
Size : 96Tx50
Brand : Abbkine
Specification
| Product name | Mouse Nesprin-2 (SYNE2) ELISA Kit |
| Reactivity | Mouse |
| Applications | ELISA |
| Applications notes | This Mouse Nesprin-2 (SYNE2) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNE2 in samples. An antibody specific for SYNE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNE2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | SYNE2; DKFZp434H2235; DKFZp686E01115; DKFZp686H1931; EDMD5; FLJ11014; FLJ43727; FLJ45710; FLJ46790; KIAA1011; NUA; NUANCE; Nesprin-2; SYNE-2; nesprin 2; nucleus and actin connecting element; synaptic nuclei |
Product Properties
| Kit components | • Mouse Nesprin-2 microplate • Mouse Nesprin-2 standard • Mouse Nesprin-2 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Mouse Nesprin-2 (SYNE2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYNE2. No significant cross-reactivity or interference between Mouse SYNE2 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
| Background | SYNE2 is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. SYNE2 contains multiple spectrin repeats and a 60-amino acid C-terminal region homologous to the Drosophila protein Klarsicht. RT-PCR analysis showed low to moderate expression of 2 SYNE2 splice variants in most tissues examined, including skeletal and cardiac muscle, kidney, liver, pancreas, and placenta, and indicated that alternative splicing patterns are conserved between SYNE1 and SYNE2.Nesprin-2-gamma also has a bipartite nuclear localization signal and a leucine zipper motif. |
| Gene ID | 319565 |
| Alternative | SYNE2; DKFZp434H2235; DKFZp686E01115; DKFZp686H1931; EDMD5; FLJ11014; FLJ43727; FLJ45710; FLJ46790; KIAA1011; NUA; NUANCE; Nesprin-2; SYNE-2; nesprin 2; nucleus and actin connecting element; synaptic nuclei |
| Accession | Q6ZWQ0 |
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