Mouse Monoclonal Anti-Desmoplakin 1/2, supernatant [Clone DP447]
Cat# 651155
Size : 5mL
Brand : Progen
| Quantity | 5 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Hybridoma cell culture supernatant |
| Reactivity | Bovine, Chicken, Human, Mouse, Trout, Zebrafish |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | DP447 |
| Immunogen | Synthetic peptide of human desmoplakin 2 (aa 2115-2132) |
| Formulation | Contains 0.09% sodium azide |
| UniprotID | A0A3Q1MR22 (Bovine),E1BWI0 (Chicken),P15924 (Human),E9Q557 (Mouse) |
| Synonym | Desmoplakin, DP, 250/210 kDa paraneoplastic pemphigus antigen, DSP |
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | Ready-to-use |
| Immunohistochemistry (IHC) - paraffin | Ready-to-use (microwave treatment recommended) |
| Western Blot (WB) | Assay dependent |
The antibody shows distinct punctate membrane staining of different epithelia. Area compositae of the myocardium of higher vertebrates are also positive. The epitope is located in a region highly conserved among species (human, zebrafish).
Polypeptides reacting: Desmoplakin 1 and 2.
Reactivity on cultured cell lines: Several human carcinoma cell lines: MCF-7, A-431, HaCaT, CaCo2; bovine cells: MDBK, BMGE; RTG-2 (rainbow trout).
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