Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit
Detection principle
Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm.
Performance characteristics
| Sample type | animal tissue |
| Sensitivity | 3.0 U/L |
| Detection range | 3.0–88.6 U/L |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 10 min |
| Precision | Average inter-assay CV: 9.500%Average intra-assay CV: 5% |
| Other instruments required | Centrifuge |
| Other reagents required | Anhydrous ethanol, PBS(0.01 M，pH 7.4) |
| Storage | -20℃ |
| Valid period | 12 months |
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits