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KLN-205 genomic DNA - 5 microgram

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Cat# 400419GD5

Size : 5ug

Brand : CLS Cell Lines Service

KLN-205 genomic DNA - 5 microgram

KLN-205 Cells

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Content: 1 cryovial

excl. taxes plus shipping costs

Product number: 400419

Safety data sheet

Description	KLN-205 is a murine lung carcinoma cell line derived from an adult mouse. This cell line is widely used in cancer research, particularly for studying the mechanisms of lung cancer progression, metastasis, and potential therapeutic interventions. KLN-205 cells exhibit characteristics typical of non-small cell lung carcinoma (NSCLC), making them a valuable model for investigating the molecular and cellular underpinnings of this disease. Researchers utilize KLN-205 to evaluate the efficacy of various chemotherapeutic agents, immunotherapies, and targeted treatments, helping to advance the understanding of lung cancer biology and treatment strategies. KLN-205 cells are known for their robust growth and ability to form tumors when implanted in immunocompromised mice, providing a reliable in vivo model for preclinical studies. These cells are used to explore tumor-host interactions, immune responses to lung cancer, and the impact of genetic and epigenetic modifications on cancer development and progression. The KLN-205 cell line serves as a critical tool in oncology research, aiding in the identification of novel biomarkers and therapeutic targets for lung cancer.
Organism	Mouse
Tissue	Lung
Disease	Squamous cell carcinoma
Synonyms	KLN 205, KLN205

Growth properties	Adherent

Citation	KLN-205 (Cytion catalog number 400419)
Biosafety level	1

Tumorigenic	Yes, in DBA/2 and BDF1 mice

Culture Medium	EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c)
Medium supplements	Supplement the medium with 10% FBS
Passaging solution	Accutase
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypLE Express (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 degree Celsius for 10 -15 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split ratio	A ratio of 1:5 is recommended
Fluid renewal	2 to 3 times per week
Freezing recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Optionally, skip centrifugation but remove any remaining freezing medium after 24 hours. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.