Ki67

Cat# NB-26-01983

Size : 100ul

Brand : Neo Biotech

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Product Details
Applications: ICC, WB, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:1,000-5,000; Western Blot: 1:5,000
Immunogen: Mixture of recombinant human Ki67 constructs expressing the N-terminal region, amino acids 1-300 and an internal region, amino acids 1111-1490
Storage: Store at 4°C.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat
Entrez:
UniProt:
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with chicken pAb to Ki67, CH22135, dilution 1:2,000 in red, and mouse mAb to β-tubulin in green. The blue is DAPI staining of nuclear DNA. The CPCA-Ki67 antibody detects Ki67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67 forms a coat around condensed chromosomes. Ki67 is not detected in cells in the quiescent G0 state.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with chicken pAb to Ki67, CH22135, dilution 1:2,000 in red, and mouse mAb to β-tubulin in green. The blue is DAPI staining of nuclear DNA. The CPCA-Ki67 antibody detects Ki67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67 forms a coat around condensed chromosomes. Ki67 is not detected in cells in the quiescent G0 state.

Western Blot

Western blot analysis of equal amounts of cell lysates probed with chicken pAb to Ki67, CH22135, dilution 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of molecular weight 345kDa and 395kDa.

Western Blot

Western blot analysis of equal amounts of cell lysates probed with chicken pAb to Ki67, CH22135, dilution 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of molecular weight 345kDa and 395kDa.