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> JC-1 [3520-43-2]

JC-1 [3520-43-2]

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Cat# 10-1634-5mg

Size : 5mg

Brand : Focus Biomolecules

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JC-1 | Mitochondrial membrane potential probe

CAS:
3520-43-2
Catalog Number:
10-1634
Activity:
Mitochondrial membrane potential probe
Chemical Name:
5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide
Molecular Weight:
652.23
Molecular Formula:
C25H27Cl4IN4
Solubility:
Soluble in DMSO (20 mg/ml)
Physical Properties:
Brown solid
Purity:
98% by HPLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Store as supplied desiccated at -20°C for up to 2 years from the date of purchase. Solutions in DMSO may be stored at -20°C for up to 3 months.
Shipping Code:
RT

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1 mg$55.00
5 mg$200.00
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JC-1 (CAS 3520-43-2) is a dual emission, potential-sensitive, carbocyanine probe which can be used to measure mitochondrial membrane potential.  When excited at 490 nm the monomer exhibits an emission maximum at 527 nm and J-aggregates at 590 nm.1,2  JC-1 changes color reversibly from green to red with increasing mitochondrial membrane potential.3  May be used for isolation of live cells expressing high multidrug resistance transport activity.4  A widely used probe for determination of mitochondrial membrane potential.5

References/Citations:

1) Reers et al. (1991), J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential; Biochemistry, 30 4480
2) Smiley et al. (1991), Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1; Proc. Natl. Acad. Sci. USA, 88 3671
3) Lisa et al. (1995), Mitochondrial membrane potential in single living adult rat cardiac myocytes exposed to anoxia or metabolic inhibition; J. Physiol., 486 1
4) Wolosin et al. (2017), Application of JC1 for non-toxic isolation of cells with MDR transporter activity by flow cytometry; PLoS One, 12(4) e0174905
5) Wang et al. (2017), A novel cytoprotective peptide protects mesenchymal stem cells against mitochondrial dysfunction and apoptosis induced by starvation via Nrf2/Sirt3/FoxO3a pathway; J. Transl. Med., 15 33

CAS:
3520-43-2
Catalog Number:
10-1634
Activity:
Mitochondrial membrane potential probe
Chemical Name:
5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide
Molecular Weight:
652.23
Molecular Formula:
C25H27Cl4IN4
Solubility:
Soluble in DMSO (20 mg/ml)
Physical Properties:
Brown solid
Purity:
98% by HPLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Store as supplied desiccated at -20°C for up to 2 years from the date of purchase. Solutions in DMSO may be stored at -20°C for up to 3 months.
Shipping Code:
RT

Safety Data Sheet:

N/A

Product Data Sheet:

Download Product Data Sheet

Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications. Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a restocking/refund fee

JC-1 (CAS 3520-43-2) is a dual emission, potential-sensitive, carbocyanine probe which can be used to measure mitochondrial membrane potential.  When excited at 490 nm the monomer exhibits an emission maximum at 527 nm and J-aggregates at 590 nm.1,2  JC-1 changes color reversibly from green to red with increasing mitochondrial membrane potential.3  May be used for isolation of live cells expressing high multidrug resistance transport activity.4  A widely used probe for determination of mitochondrial membrane potential.5

References/Citations:

1) Reers et al. (1991), J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential; Biochemistry, 30 4480
2) Smiley et al. (1991), Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1; Proc. Natl. Acad. Sci. USA, 88 3671
3) Lisa et al. (1995), Mitochondrial membrane potential in single living adult rat cardiac myocytes exposed to anoxia or metabolic inhibition; J. Physiol., 486 1
4) Wolosin et al. (2017), Application of JC1 for non-toxic isolation of cells with MDR transporter activity by flow cytometry; PLoS One, 12(4) e0174905
5) Wang et al. (2017), A novel cytoprotective peptide protects mesenchymal stem cells against mitochondrial dysfunction and apoptosis induced by starvation via Nrf2/Sirt3/FoxO3a pathway; J. Transl. Med., 15 33

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10-1634-1mg
JC-1 [3520-43-2]
 1mg