JC-1 [3520-43-2]
Cat# HY-15534-5mg
Size : 5mg
Brand : MedChemExpress
JC-1 (CBIC2) is an ideal fluorescent probe widely used to detect mitochondrial membrane potential. JC-1 accumulates in mitochondria in a potential dependent manner and can be used to detect the membrane potential of cells, tissues or purified mitochondria. In normal mitochondria, JC-1 aggregates in the mitochondrial matrix to form a polymer, which emits strong red fluorescence (Ex=488 nm, Em=595 nm); When the mitochondrial membrane potential is low, JC-1 cannot aggregate in the matrix of mitochondria and produce green fluorescence (ex=488 nm, em= 530 nm).
For research use only. We do not sell to patients.
JC-1 Chemical Structure
CAS No. : 3520-43-2
This product is a controlled substance and not for sale in your territory.
Based on 118 publication(s) in Google Scholar
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JC-1 purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Aug 11;21:20-31. [Abstract]
- JC-1 (20 min at 37 °C in dark) staining is used to analyze mitochondrial membrane in H9C2 cells.
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JC-1 purchased from MedChemExpress. Usage Cited in: ACS Nano. 2022 Mar 28. [Abstract]
- To investigate the mitochondrial membrane potential, ACCO is stained with JC-1 for 30 min, washed with PBS, and analyzed by flow cytometry using 488 nm excitation with 530/30 and 585/42 nm band-pass filters.
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JC-1 purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2022 May;179:106123. [Abstract]
- Mitochondrial membrane potential (Δψm) is measured with fluorochrome dye JC-1 (15 μM; 37 °C for 30 min in darkness). Flow cytometry is used to measure red (aggregation of JC-1) and green (monomeric JC-1) fluorescence intensity in HUVECs.
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JC-1 purchased from MedChemExpress. Usage Cited in: Small. 2021 Aug;17(32):e2101368. [Abstract]
- Laser scanning confocal fluorescence microscopy images of JC-1, LysoTracker Red DND-99, and AO staining for determining mitochondrial membrane potential, lysosomal deacidification, and lysosomal membrane permeabilization, respectively.
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JC-1 purchased from MedChemExpress. Usage Cited in: Small. 2021 Feb;17(7):e2005865. [Abstract]
- In 4T1 cells, A549 cells, and HeLa cells, cells are treated with JC-1 (5 µg/mL) for 20 min in the darkness, the fluorescence is observed by flow cytometry and the fluorescent color of the cells was observed with a fluorescence microscope, respectively
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JC-1 purchased from MedChemExpress. Usage Cited in: Small. 2021 Feb;17(7):e2005865. [Abstract]
- In 4T1 cells, A549 cells, and HeLa cells, cells are treated with JC-1 (5 µg/mL) for 20 min in the darkness, the fluorescence is observed by flow cytometry and the fluorescent color of the cells was observed with a fluorescence microscope, respectively
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JC-1 purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2019 Apr 10;18(1):85. [Abstract]
- KRA-533 induces caspase 3 activation and reduces mitochondrial membrane potential in NSCLC cells. The measurement of mitochondrial membrane potential by JC-1 staining.
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JC-1 purchased from MedChemExpress. Usage Cited in: Small. 2019 Sep;15(36):e1902642. [Abstract]
- H9C2 cells are rinsed with pre-cooled PBS for 3 times and collected in PBS buffer containing 10 µg/mL JC-1 probe. After incubation for 20 min in dark place, cells are rinsed with PBS twice by centrifugation to remove the supernatant. Finally, H9C2 cell pellets suspended in PBS are analyzed by a flow cytometric analyzer.
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JC-1 purchased from MedChemExpress. Usage Cited in: New J Chem. 2017 41(23).
- Measurement of mitochondria membrane potential by JC-1 staining. HeLa cells are incubated with or without WOs for 24 h, followed by NIR irradiation for NIR and WOs + NIR groups.
Description |
JC-1 (CBIC2) is an ideal fluorescent probe widely used to detect mitochondrial membrane potential. JC-1 accumulates in mitochondria in a potential dependent manner and can be used to detect the membrane potential of cells, tissues or purified mitochondria. In normal mitochondria, JC-1 aggregates in the mitochondrial matrix to form a polymer, which emits strong red fluorescence (Ex=488 nm, Em=595 nm); When the mitochondrial membrane potential is low, JC-1 cannot aggregate in the matrix of mitochondria and produce green fluorescence (ex=488 nm, em= 530 nm)[1]. |
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In Vitro |
JC-1 staining MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
652.23 |
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Formula |
C25H27Cl4IN4 |
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CAS No. |
3520-43-2 |
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Appearance |
Solid |
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Color |
Purple to purplish red |
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Emission (Em) |
527/590 |
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Excitation (Ex) |
490 |
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SMILES |
ClC1=C(Cl)C=C([N+](CC)=C(/C=C/C=C2N(C(C=C(Cl)C(Cl)=C3)=C3N\2CC)CC)N4CC)C4=C1.[I-] |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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Solvent & Solubility |
In Vitro:
DMSO : 5 mg/mL (7.67 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : < 0.1 mg/mL (insoluble) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation | |||||||||||||
Dyeing Example |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 1.5332 mL | 7.6660 mL | 15.3320 mL | 38.3300 mL |
5 mM | 0.3066 mL | 1.5332 mL | 3.0664 mL | 7.6660 mL |
JC-1 Related Classifications
- Others
- Others
- Fluorescent Dye