Reagents and instruments for immunology, cell biology and molecular biology.
 
> IgG, F(ab')2 (Biotin)

IgG, F(ab')2 (Biotin)

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Cat# I1904-59V-500ug

Size : 500ug

Brand : US Biological

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I1904-59V IgG, F(ab')2 (Biotin)

Clone Type
Polyclonal
Host
rabbit
Source
sheep
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications: |Suitable for use in ELISA, Western Blot, Immunomicroscopy and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|ELISA: 1:40,000-1:160,000|Optimal dilutions to be determined by the researcher.||Label: |Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)||Biotin/Protein Ratio: |10-20 BAC molecules per Rabbit IgG molecule||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: sheep|Concentration: ~1.5mg/ml (UV280)|Form: Supplied as a liquid in 0.02 M PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Sheep IgG F(ab’)2 fragment|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab’)2 and Sheep Serum. No reaction was observed against Sheep IgG F(c).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Sheep IgG F(ab’)2 fragment
Form
Supplied as a liquid in 0.02 M PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin.
Purity
Purified by immunoaffinity chromatography.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab’)2 and Sheep Serum. No reaction was observed against Sheep IgG F(c).