IgA, alpha (Biotin)

Applications: |Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested. ||Recommended Dilutions: |ELISA: 1:10,000 to 1:30,000 1.0ug of Human IgG was used in a standard capture ELISA with Streptavidin (HRP) (S7973-97A) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (A0030) as a substrate for 30 minutes at RT.|Optimal dilution determined by the researcher. ||Label: |Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)||Biotin/Protein Ratio: |10-20 BAC molecules per Goat IgG molecule||Storage and Stability:|Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.