Size : 25ml
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HyperChrom Ni-NTA Excel Agarose is an affinity chromatography medium that uses a highly cross-linked agarose gel as a matrix to incorporate a transition metal ion Ni2+ with tetracoordination, chemically coupled to a ligand of Nitrilotriacetic acid (NTA). It has a very stable octahedral structure, with nickel ions in the center of the octahedron to protect nickel ions from small molecules, is more stable, and can withstand harsh conditions such as reducing agents, denaturants or couplants at certain concentrations. In addition, due to the pressure resistance of the matrix, which can withstand pressures up to 0.3 MPa, the product can be used for industrial-scale protein purification, enabling purification of the protein of interest at relatively high flow rates. This chromatography medium uses the interaction between Ni2+ and the side chains of certain amino acids (mainly histidine) on proteins to enable the separation and purification of proteins with and without these amino acids, and with or without these amino acids.
Compared to HyperChrom Ni-NTA FF Agarose (PC2001) and HyperChrom Ni-NTA HP Agarose (PC2002), this product has the following advantages: (i) it has strong binding force with Ni2+, can tolerate 100 mM EDTA, and has better compatibility with buffers, (ii) has high alkali resistance, can withstand 0.5 M NaOH immersion for 48 h, does not need to remove nickel cleaning, avoids cross-contamination, saves time and efficiency.
HyperChrom Ni-NTA Excel Agarose chromatography media parameters
Name
Description
Chromatography media type
Affinity chromatography media
Ligands
NTA-Ni2+
Scaffolding
Highly cross-linked agarose
Average particle size
90 μm
Ligand density
54~70 μmol Ni2+/mL chromatography medium
Dynamic load
≥ 10 mg histidine-tagged protein/mL chromatography medium
Flow rate is recommended
150-600 cm/h
Maximum flow rate
600 cm/h
Pressure-resistant
0.3 MPa
Operating temperature
4 - 30℃
pH stability *
2-14
Storage
4oC for 5 years
Transport condition
Room temperature
Chemical resistance
0.1 M~0.5 M NaOH for 48 h
10 mM β-mercaptoethanol, 5 mM TCEP for 24 h
500 mM imidazole, 100 mM EDTA, 2 h
*After 7 days of chromatography media at 40°C, pH 2-14, there was no significant change in its physicochemical properties and functions.
