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> Human IgG4 monoclonal antibody, clone RM120 (Biotin)

Human IgG4 monoclonal antibody, clone RM120 (Biotin)

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Human IgG4 monoclonal antibody, clone RM120 (Biotin)

Cat# MAB14959

Size : 50ug

Brand : Abnova

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  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against of human IgG4.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs,RAB,MAB,Recombinant Mab,MAB,monoclonal Ab,monoclonal antibody

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against a synthetic peptide corresponding to the hinge region of human IgG4.

    Sequence

    N/A

    Reactivity

    Human

    Form

    Liquid

    Conjugation

    Biotin

    Purification

    Protein A purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.05-0.2 ug/well for capture, 0.05-0.2 ug/mL for detection)
    Flow Cytometry
    Immunocytochemistry (1-10 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-10 ug/mL)
    Western Blotting (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Western blot analysis of human, mouse, rat, and goat IgG with Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat # MAB14959) at 0.5 ug/mL working concentration.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat # MAB14959).

    Immunocytochemistry

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of human IgG4. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Flow Cytometry

  • Gene Info

    Entrez GeneID

    3503

    Protein Accession#

    P01861

    Gene Name

    IGHG4

    Gene Alias

    MGC117419

    Gene Description

    immunoglobulin heavy constant gamma 4 (G4m marker)

    Omim ID

    147130

    Gene Ontology

    Hyperlink

    Other Designations

    -

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