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> Human Glucagon Ready-To-Use IHC Kit

Human Glucagon Ready-To-Use IHC Kit

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Cat# IHC0104H

Size : 50Test

Brand : Bioss

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Human Glucagon Ready-To-Use IHC Kit

Immunohistochemical analysis of paraffin embedded human pancreas tissue slide using IHC0104H (Human Glucagon IHC Kit).

Human Glucagon Ready-To-Use IHC Kit

Cat. No.: IHC0104H
Sample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Background

Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogensis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide residue almost exclusively with the exsistence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as a cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in qualification of the hormone.

Synonyms

GCG; Glicentin related polypeptide; GLP 1; GLP 2; GLP1; GLP2; Glucagon like peptide 1; Glucagon like peptide 2; Glucagon precursor; GRPP; GLUC_HUMAN.

materials Supplied

Number Component 50T Concentration Storage
1 PBS Buffer (powder) 2L x 2 20x RT
2 Antigen Retrieval Buffer 20ml 100x 2-8°C
3 Endogenous Peroxidase Blocking Buffer 3ml RTU 2-8°C
4 Blocking Buffer 3ml RTU 2-8°C
5 Primary Antibody (Human Glucagon Rabbit pAb) 6ml RTU 2-8°C
6 Secondary Antibody (HRP-Goat anti-Rabbit IgG pAb) 6ml RTU 2-8°C
7 Chromogen Component A 0.3ml RTU -20°C
8 Chromogen Component B 0.3ml RTU -20°C
9 Counter Staining Reagent 5ml RTU RT
10 Differentiation Reagent 6ml RTU RT
11 Mounting Media 5ml RTU RT
12 Control slide (Human pancreas) 1 slide RTU RT
13 Datasheet 1 copy

Notes

  1. The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
  2. Do not allow slides to dry at any time during this procedure.
  3. Please don't replace the matching reagents in this product with other manufacturers' products.
  4. As DAB is a carcinogen, please take necessary precautions.
  5. PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.

Please cite this product as "IHC0104H, Bioss Antibodies".
Citation example: “Human tissue sections using Human Glucagon IHC Kit (IHC0104H, Bioss Antibodies) were stained for Glucagon according to the manufacturer's instructions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.