Reagents and instruments for immunology, cell biology and molecular biology.
 
> Human GDF11(Growth Differentiation Factor 11) ELISA Kit

Human GDF11(Growth Differentiation Factor 11) ELISA Kit

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Cat# E-EL-H1908-96T

Size : 96T

Brand : Elabscience


Product Summary
Sensitivity 9.38 pg/mL
Detection Range 15.63-1000 pg/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Human
Specificity This kit recognizes Human GDF11 in samples. No significant cross-reactivity or interference between Human GDF11 and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃/-20℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDF11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GDF11. You can calculate the concentration of Human GDF11 in the samples by comparing the OD of the samples to the standard curve.
Uniport ID O95390
Research Area Cell Biology , Neuroscience , Signal Transduction
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