HotBegan™ Hot Start Taq-DNA Polymerase, MasterMix
Cat# P0030
Size : 2x1.25mL(250rxn)
Brand : Canvax Biotech
HotBegan™ Hot Start Taq-DNA Polymerase, MasterMix
For a Specific, Highly Efficient & Reliable Amplifications of DNA up 160 fg in Ready-to-use Format
HotBegan™ Hot Start Taq DNA Polymerase MasterMix an optimized ready-to-use solution containing HotBegan™ Taq DNAPolymerase (hot start performance), dNTPs, MgCl2 and Stabilizers. It is inactive at Room Temperature and includes all material needed except Template and Primers.
It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products.
Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.
Detailed information:
- Time-saving solution: ready-to-use MasterMix.
- High specificity: minimizes unspecific amplification.
- Efficient: prevents or minimizes primer-dimer and nonspecific products.
- Great sensitivity: amplifies from femptograms of DNA targets.
- Inactive: at Room Temperature.
- Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments.
- Powerful: amplification of targets up to 5 kb.
- Convenient: available in kits and MasterMix solution.
Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.
– 2 x 1.25 mL HotBegan™ Hot Start Taq DNA Polymerase MasterMix, which includes HotBegan™ Hot Start Taq DNA Polymerase (0.1 U/μL), 2x PCR Buffer, 0.4 mM of each dNTP, 4 mM Mg2+, 4% Glycerol.
– 2 x 1.25 mL Nuclease-free water.
Datasheet MSDS- Real time PCR.
- RT-PCR and quantitative RT-PCR.
- Genotyping with Taqman Probes.
- PCR fragments amplification for TA or GC Cloning.
- Amplification from a limited DNA template or low copy number genes.
- Functionally tested in qPCR.
- None detected bacterial DNA (by qPCR).
- Exempt of nucleases (endo-, exo and ribonucleases) activity guaranteed by appropriate quality tests.
- Shipped in: Gel pack.
- Storage: -20 °C.
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This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.