Reagents and instruments for immunology, cell biology and molecular biology.
 
> Hoechst 33342

Hoechst 33342

Print Print
Please log in

Cat# 40047

Size : 100MG

Brand : Biotium

Request more information

Hoechst

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains.

Hoechst 33342, 10 mg/mL in H2O
Hoechst 33258, 10 mg/mL in H2O
Hoechst 33258, Pentahydrate

Product Attributes

CAS number

23491-45-4, 23491-52-3
Detection method/readout

Fluorescence microscopy, Flow cytometry
Probe cellular localization

Nucleus
For live or fixed cells

For fixed cells, For live/intact cells
Assay type/options

DNA content/cell cycle profiling by flow cytometry, Long term staining (24-72h), No-wash staining, Real-time imaging, Tissue staining
Cell permeability

Membrane permeant
Apoptosis/viability marker

All cell stain
Fixation options

Fix before staining (formaldehyde), Fix after staining (formaldehyde), Fix before staining (methanol), Fix after staining (methanol), Permeabilize after staining
Colors

Blue
Excitation/Emission

352/458 nm (with DNA)

Technology

Cellular Stains
Considerations for Immunofluorescence Staining

Tech Tips

Hoechst & DAPI Staining Protocols – Cell Staining with Hoechst or DAPI Nuclear Stains
Protocols
SDS
Supporting Documents

Product Description

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains. Biotium offers Hoechst 33258 and Hoechst 33342 dyes in both solution or powder forms. Both dyes are spectrally similar but Hoechst 33258 is slightly more water soluble than Hoechst 33342. The dyes can be used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.

Hoechst can also be used to stain live bacteria (gram-positive and gram-negative), but in live yeast the staining is weak and not nuclear. Check out our tech tip on Hoechst & DAPI Staining, which includes helpful tips and detailed protocols for staining live and fixed cells and tissues. Also see our Cellular Stains Table for more information on how our dyes stain various organisms.

Hoechst 33258, pentahydrate

 

Hoechst 33342, trihydrochloride trihydrate

Biotium also offers unique NucSpot® Nuclear Stains for bright and specific nuclear staining in dead or fixed cells. The stains are available in a wide range of colors from green to near-IR. We also offer DAPI nuclear stains in powder or solution.

Hoechst Products

Product Catalog Number MW Unit Size λEx/λEm Format
Hoechst 33258, 10 mg/mL in Water 40044 533.88 10 mL 352/458 nm Yellow solution
Hoechst 33258, pentahydrate 40045 623.96 100 mg 352/458 nm Yellow solid
Hoechst 33342, 10 mg/mL in Water 40046 561.93 10 mL 350/461 nm Yellow solution
Hoechst 33342, trihydrochloride trihydrate 40047 615.98 100 mg 350/461 nm Yellow solid

Having trouble with your experiment? See our section on troubleshooting tips for fluorescent staining.

 

You might also be interested by the following products:



Cat#
Description
Cond.
Price Bef. VAT