Reagents and instruments for immunology, cell biology and molecular biology.
 
> Histone H1 Polyclonal Antibody

Histone H1 Polyclonal Antibody

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Cat# E-AB-31676-20

Size : 20uL

Brand : Elabscience

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Verified Samples Verified Samples in WB: 3T3
Dilution WB 1:500-1:2000,  IHC 1:100-1:300,  IF 1:200-1:1000
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse
Applications WB,  IHC-p,  IF
Clonality Polyclonal
Immunogen Synthesized peptide derived from human Histone H1 around the non-phosphorylation site of Thr17.
Abbre Histone H1
Synonyms H1F3,  H1F4,  H1F5,  HIST1H1B,  HIST1H1D,  HIST1H1E,  Histone H1.3,  Histone H1.4,  Histone H1.5,  Histone H1a,  Histone H1b,  Histone H1c,  Histone H1s-2,  Histone H1s-3,  Histone H1s-4
Swissprot
Calculated MW 23 kDa
Observed MW 31 28kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization Extracellular region or secreted, extracellular exosome, Nucleus, nuclear heterochromatin, nuclear nucleosome, nucleus.
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification Method Affinity purification
Research Areas Cancer,  Epigenetics and Nuclear Signaling
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
Cat.No. Product Name Sizes
E-AB-1003 Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1010 Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated) 500μL , 120μL , 60μL
E-AB-1014 Goat Anti-Rabbit IgG(H+L)(FITC conjugated) 500μL , 120μL , 60μL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1060 Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 594 conjugated) 500μL , 120μL , 60μL
E-AB-1075 Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 647 conjugated) 500μL , 120μL , 60μL
E-AB-1099 Elab Fluor® Violet 450-Goat Anti-Rabbit IgG(H+L) 500μL , 120μL , 1mL
E-AB-1194 HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R217 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) 50mL , 18mL , 6mL , 3mL
E-IR-R220 Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) 10mL , 3mL
E-IR-R221 Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) 10mL , 3mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Unconjugated

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