Glycerol Colorimetric Assay Kit
Cat# E-BC-K340-M-96T
Size : 96T
Brand : Elabscience
Detection Principle | Glycerol is transformed by enzyme to produce hydrogen peroxide, which is catalyzed by oxidase in the presence of 4-amino-antipyrprid and phenol to produce red quinones, the color of which is proportional to the content of glycerol. |
Sample Type | Serum,Plasma,Animal tissue,Cell |
Detection Method | Colorimetric method |
Detection Instrument | Microplate reader (500-520 nm, optimum wavelength: 510 nm) |
Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
Other Reagents Required | Double distilled water, Isopropanol (AR), PBS (0.01 mol/L, pH 7.4) |
Storage | This product can be stored at 2-8°C for 12 months with shading light. |
Valid Period | 12 months |
Sensitivity | 0.01 mmol/L |
Detection Range | 0.01-1.00 mmol/L |
Precision | inter-assay CV: 0.08 | intra-assay CV: 0.04 |
Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
10% Mouse liver tissue homogenate | 2 |
10% Mouse kidney tissue homogenate | 1 |
10% Mouse spleen tissue homogenate | 1 |
Cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor®405
Elab Fluor®430
Elab Fluor®488
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