• Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of Flt1.

    Immunogen

    A synthetic peptide corresponding to residues within 800-900 at internal region of mouse Flt1.

    Host

    Rabbit

    Reactivity

    Human

    Specificity

    This antibody targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein. This antibody is useful for Western blot, where a band is seen at ~180-230 kDa (the theoretical molecular weight of mouse VEGFR1 is ~150 kDa and human VEGFR1 is ~151 kDa). The difference in MWs is likely due to glycosylation or other post translational modifications. With CSF-1R/VEGFR2 chimera transfected lysates a doublet is seen at ~150 kDa representing VEGFR2. An unknown band is also seen at ~50 kDa.

    Form

    Liquid

    Conjugation

    DyLight 650

    Purification

    Immunogen affinity purification

    Recommend Usage

    Immunocytochemistry/Immunofluorescence
    Immunohistochemistry
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Western Blot
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In 50 mM sodium borate (0.05% sodium azide).

    Storage Instruction

    Store at 4°C in the dark.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry

    Immunocytochemistry

    Immunofluorescence

  • Gene Info — Flt1

    Entrez GeneID

    14254

    Protein Accession#

    P35969

    Gene Name

    Flt1

    Gene Alias

    AI323757, Flt-1, VEGFR-1, VEGFR1, sFlt1

    Gene Description

    FMS-like tyrosine kinase 1

    Gene Ontology

    Hyperlink

    Other Designations

    OTTMUSP00000023727|vascular endothelial growth factor receptor|vascular endothelial growth factor receptor-1|vascular permeability factor receptor