Reagents and instruments for immunology, cell biology and molecular biology.
 
> DyLight 350, Goat Anti-Rabbit IgG

DyLight 350, Goat Anti-Rabbit IgG

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Cat# A23020-500

Size : 500uL

Brand : Abbkine

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Specification

Product name DyLight 350, Goat Anti-Rabbit IgG
Immunogen Rabbit IgG whole molecule
Host Goat
Reactivity Rabbit
Applications FCM, ICC, IF
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.
Clonality Polyclonal
Preparation method Affinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE
Conjugate Dylight 350

Product Properties

Formulation Liquid solution
Features & Benefits DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.
Storage instructions Stable for one year at -20 class="pro-form-title">Additional Information
Background Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.
Others The antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.

Image & description

Fig.3. To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488, a green (526 and 594, and a red (633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700. To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488, a green (526 and 594, and a red (633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 class=This product has been cited in4publications