Product Description  

The Dual Luciferase Reporter Assay Kit is used to detect gene regulation by transfecting cells with a reporter plasmid and measuring the fluorescence intensity of Luciferin substrate to reflect the level of Luciferase expression. To achieve dual luciferase reporter gene detection, Firefly luciferase is detected with Luciferin as a substrate, and Renilla Iuciferase is detected with coelenterazine as a substrate, while inhibiting the activity of Firefly luciferase. Dual Luciferase Reporter Assay Kit can detect the expression of Luciferase regulated by gene elements sensitively and efficiently. Usually, the transcriptional regulatory element is cloned upstream of Firefly luciferase, or the 3'-UTR regulatory region is cloned downstream of firefly luciferase. The transfected cells are induced by corresponding stimulator and lysed to determine the luciferase activity. The stimulatory-inducing effect of the regulatory elements is evaluated by luciferase activity. Renilla Iuciferase acts as an internal reference for correcting transfection efficiency to eliminate differences in cell number and transfection efficiency between wells. Firefly luciferase catalyzes the emission of Luciferin at 560 nm, and Renilla luciferase catalyzes the emission of coelenterazine at 465 nm.

 Features  

• Strong fluorescent signal: Suitable for the detection of weak promoters or low-expression regulation

• Wide detection range: Linear range up to 8 orders of magnitude (R²>0.99)

• High sensitivity: Detection sensitivity as low as 10-18 mol

 Components  

 Storage  

Store at -30 ~ -15°C and transport at ≤0°C. Dissolved and dispensed Reagent: long-term storage at -85 ~ -65°C, or short-term storage at -30 ~ -15°C for no more than one month; Stop & Reaction Buffer: long-term storage at 2 ~ 8°C.