Donkey anti-Rabbit IgG (H&L), DyLight® 650 conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
Cat# AS122329
Size : 1mg
Brand : Agrisera
Donkey anti-Rabbit IgG (H&L), DyLight® 650 conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
AS12 2329 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rabbit IgG (H&L)
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Immunogen: | Purified Rabbit IgG, whole molecule |
Host: | Donkey |
Clonality: | Polyclonal |
Purity: | Immunogen affinity purified donkey IgG. |
Format: | Lyophilized |
Quantity: | 1 mg |
Reconstitution: | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily |
Storage: | Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
Recommended dilution: | 1 : 20-1 : 2000 for most applications |
Additional information: | Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1% (w/v) BSA, Protease/IgG free. 0.05% (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins No reactivity is observed to: non-immunoglobulin rabbit serum proteins, IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep |
Background: | Donkey anti-Rabbit IgG (H&L) - DyLight®650 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight® 650, which binds to Rabbit IgG (H&L) in immunological assays. DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are purified using solid phase Rabbit IgG DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries. |