Contains 11 discrete fragments ranging from 50bp to 1000bp with a double intensity reference band at 500bp. Suitable for size determination of digested DNA.||The marker yields the following 11 discrete fragments: 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 50.||Quantity of DNA in band:||Fragment (bp) ng/10ul|1000 91|900 66|800 61 |700 51 |600 43 |500 70 |400 28 |300 23|200 30|100 22|50 15||Recommendations for Use:|1. Vortex gently just prior to use.|2. Do not heat before loading.|3. Apply 10ul of DNA Ladder per well.|4. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5ug/ml ethidium bromide for 30-40 minutes. ||*Note: This ladder was standardized using 10ul of DNA per lane on a 0.8cm thick, 13x15cm, 1% agarose gel rrun in TAE buffer.||Storage and Stability:|May be stored at -20°C For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Important Note: |This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. ||Toxicity and Hazards: |All products should be handled by qualified personnel only, trained in laboratory procedures.