Images
Western Blot (Tissue lysate)
Application

Western Blot (Tissue lysate)

Western Blot (Tissue lysate) analysis of bovine tissue lysates. Lane 1: Alpha A Crystallin; Lane 2: Alpha B Crystallin

Immunofluorescence
Application

Immunofluorescence

Immunofluorescence staining of SK-N-BE. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Alpha A Crystallin Antibody (D) Composite.

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against native human CRYAA.

    Immunogen

    Native purified human CRYAA.

    Host

    Mouse

    Reactivity

    Bovine, Human, Mouse, Rat

    Specificity

    This antibody detects ~20 kDa. Does not cross-react with alpha subunit B-crystallin, beta subunit L-crystallin, beta subunit H-crystallin, gamma subunit crystallin, HSP25, HSP27 or HSP47 proteins.

    Form

    Liquid

    Conjugation

    RPE

    Purification

    Protein G purification

    Isotype

    IgG1

    Recommend Usage

    Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Western Blot (1:2000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.2 (50% glycerol, 0.09% sodium azide).

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    Application Data with Unconjugated Antibody.
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Tissue lysate)

    Western Blot (Tissue lysate) analysis of bovine tissue lysates. Lane 1: Alpha A Crystallin; Lane 2: Alpha B Crystallin

    Immunocytochemistry

    Immunofluorescence

    Immunofluorescence staining of SK-N-BE. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Alpha A Crystallin Antibody (D) Composite.
  • Gene Info — CRYAA

    Entrez GeneID

    1409

    Protein Accession#

    P02489

    Gene Name

    CRYAA

    Gene Alias

    CRYA1, HSPB4

    Gene Description

    crystallin, alpha A

    Omim ID

    123580

    Gene Ontology

    Hyperlink

    Gene Summary

    Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq

    Other Designations

    crystallin, alpha-1|human alphaA-crystallin (CRYA1)

  • Interactomes
  • Diseases