Reagents and instruments for immunology, cell biology and molecular biology.

Creatine kinase (CK) Activity Assay Kit

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Cat# E-BC-K558-S-100

Size : 100Assays

Brand : Elabscience

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Detection Principle	Creatine kinase (CK) catalyze creatine phosphate and ADP to produce creatine and ATP. Hexokinase catalyze creatine and glucose to produce glucose-6-phosphate. Glucose-6-phosphate dehydrogenase (G-6-PD) catalyze glucose-6-phosphate and NADP⁺ to produce NADPH which have a maximum absorption peak at 340 nm. The CK activity can be calculated by measuring the OD values at 340 nm.
Synonyms	CK;Creatine phosphokinase
Sample Type	Serum,Plasma,Animal tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (340 nm)
Research Area	Amino Acids And Proteins
Other Reagents Required	PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	3.7 U/L
Detection Range	3.7-160 U/L
Precision	inter-assay CV: 8.1% \| intra-assay CV: 5.2%
Assay Time	1 h 20 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Human plasma	1
Mouse serum	1
Rat serum	1
HepG2 cells homogenization	1
10% Rat kidney tissue homogenization	1
10% Rat brain tissue homogenization	2-5
10% Rat liver tissue homogenization	2-10

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.