Reagents and instruments for immunology, cell biology and molecular biology.

Cholinesterase (CHE) Activity Assay Kit

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Cat# E-BC-K052-S-100

Size : 100Assays

Brand : Elabscience

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Detection Principle	Cholinesterase breaks down acetylcholine into choline and acetic acid. Acetylcholine that is not hydrolyzed by cholinesterase reacts with basic hydroxylamine to form acetamidamine. It reacts in an acidic solution to form a brown-red hydroxamate iron complex. The color depth is directly proportional to the amount of remaining acetylcholine, which can be colorimetrically quantified. Cholinesterase activity was calculated.
Synonyms	CHE
Sample Type	Whole blood,Serum,Plasma,Tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (520 nm)
Research Area	Liver And Renal Function
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.17 U/mL
Detection Range	1.17-160 U/mL
Precision	inter-assay CV: 9.4% \| intra-assay CV: 3.7%
Assay Time	1 h

Cat.No.	Product Name	Sizes
E-BC-K125-S	Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)	100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	2-3
Human plasma	2-3
Mouse serum	2-3
Mouse plasma	2-3
SH-SY5Y cells	1
10% Mouse brain tissue homogenate	1
10% Rat brain tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Mouse liver tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.