Reagents and instruments for immunology, cell biology and molecular biology.

CD8 (Biotin)

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Cat# 214734-Biotin-100ul

Size : 100ul

Brand : US Biological

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214734-Biotin CD8 (Biotin)

Clone Type

Polyclonal

Host

mouse

Source

bovine

Swiss Prot

P31783

Isotype

IgG2a

Grade

Affinity Purified

Applications

FC IF IHC IP

Crossreactivity

Bo Gt Sh

Shipping Temp

Blue Ice

Storage Temp

-20°C

Reacts with the bovine CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of 34kD and 38kD under reducing conditions. CD8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrez et al. 1999).||Applications: |Suitable for use in Immunofluorescence, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Immunohistochemistry: Frozen and paraffin sections|Optimal dilutions to be determined by the researcher.||Hybridoma: |NS1 myeloma cells with spleen cells from BALB/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG2a|Clone No: CC63|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant|Specificity: Recognizes bovine CD8. Species Crossreactivity: goat and sheep||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant

Specificity

Recognizes bovine CD8. Species Crossreactivity: goat and sheep

References

1. MacHugh. N. et al. (1991) Individual antigens of cattle. Bovine CD8 (Bo CD8). Vet. Immunol. Immunopathol. 27:65-69. 2. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.Vet. Immunol. Immunopathol. 71: 321-334. 3. Twizere, J. et al. (2000) Discordance between bovine leukemia virus tax immortalization in vitro and oncogenicity in vivo. J. Virol. 74: 9895-9902. 4. Winkler, M. et al. (1999) Bovine Herpesvirus 1 Can Infect CD4+ T Lymphocytes and Induce Programmed Cell Death during Acute Infection of Cattle.J. Virol. 73: 8657-8668. 5. Winkler, M. et al. (2000) Persistence and Reactivation of Bovine Herpesvirus 1 in the Tonsils of Latently Infected Calves. J. Virol. 74: 5337-5346. 6. Sidders, B. et al. (2008) Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosis complex.Infect Immun. 76: 3932-9. 7. Sanchez, J. et al. (2011) Microscopical and Immunological Features of Tuberculoid Granulomata and Cavitary Pulmonary Tuberculosis in Naturally Infected Goats.J Comp Pathol. Feb 17. [Epub ahead of print] 8. La Manna, M.P. et al. (2011) Expansion of intracellular IFN-? positive lymphocytes during Mycoplasma agalactiae infection in sheep.Res Vet Sci. Feb 25. [Epub ahead of print]. 9. Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.J Virol. 80: 7873-84. 10. Harris, J. et al. (2002) Expression of caveolin by bovine lymphocytes and antigen-presenting cells.Immunology. 105: 190-5. 11. Liebana, E. et al. (2007) Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph-node granulomas.Vet Pathol. 44: 366-72. 12. Lynch, E.M. et al. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves.BMC Vet Res. 6: 39. 13. Lacroux, C. et al. (2011) Prionemia and leuco-platelet associated infectivity in sheep TSE models.J Virol. Dec 7. [Epub ahead of print] 14. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.Vet Res. 41: 14. 15. Toman, M. et al. (2003) Immunological characteristics of ca??le with Mycobacterium avium subsp. paratuberculosis infectionVet. Med.-Czech, 48, 2003: 147-54. 16. Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.Int J Parasitol. 40: 865-75.