Calcium Assay Kit (Red Fluorescence)
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- More Files
A calcium standard curve is shown with 96-well black plate and the Calcium Assay Kit (Red Fluorescence) (Cat # KA4080). As low as 0.01 mM calcium can be detected with 5 minutes incubation time (n=3). The fluorescence in blank wells (with H2O only) is used as a control, and is subtracted from the values for those wells with calcium reactions. Note: The fluorescence background increases with time, thus it is important to subtract the fluorescence intensity value of the blank wells for each data point.
- Specifications
Product Description
Calcium Assay Kit (Red Fluorescence) is used for the measurement of calcium level in physiology solutions.
Suitable Sample
Physiological Solutions
Sample Volume
50 uL
Excitation/<br>Emission (nm)
540/590 nm
Detection Method
Fluorometric
Instrument Platform
Microplate Reader
Calibration Range
0.003 to 3 mM
Limit of Detection
0.03 mM
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit in desiccated environment at -20°C and avoid from light.
- Applications
Quantification