Reagents and instruments for immunology, cell biology and molecular biology.
 
> Anti-SARS-CoV-2 Nucleoprotein antibody (1-420)

Anti-SARS-CoV-2 Nucleoprotein antibody (1-420)

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Cat# NB-22-47911-100

Size : 100uL

Brand : Neo Biotech

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  • Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein, using SARS-CoV-2 Nucleoprotein antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of Recombinant Nucleoprotein of Coronavirus SARS-CoV-2, using SARS-CoV-2 Nucleoprotein antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immobilized Recombinant SARS-COV-2 Nucleocapsid (RP01264) at 1 Mu g/mL (100 Mu L/well) can bind SARS-CoV-2 Nucleoprotein Antibody with a linear range of 3. 12-200ng/mL.
  • Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 Nucleoprotein rabbit polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Nucleoprotein antibody . Western blot was performed from the immunoprecipitate using SARS-CoV-2 Nucleoprotein antibody at a dilution of 1:1000.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Nucleoprotein antibody . Western blot was performed from the immunoprecipitate using SARS-CoV-2 Nucleoprotein antibody at a dilution of 1:3000.

General Info

Host: Rabbit
Applications: WB/IF/IP
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2 Nucleoprotein (1-420) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
DB 1:500-1:2000
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Immunogen Region: 1-420
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-420 of coronavirus Nucleoprotein (P0DTC9).
Immunogen Sequence: MSDNGPQNQRNAPRITFGGP SDSTGSNQNGERSGARSKQR RPQGLPNNTASWFTALTQHG KEDLKFPRGQGVPINTNSSP DDQIGYYRRATRRIRGGDGK MKDLSPRWYFYYLGTGPEAG LPYGANKDGIIWVATEGALN TPKDHIGTRNPANNAAIVLQ LPQGTTLPKGFYAEGSRGGS QASSRSSSRSRNSSRNSTPG SSRGTSPARMAGNGGDAALA LLLLDRLNQLESKMSGKGQ

Description

Background Enables several functions, including ATPase binding activity; calmodulin binding activity; and calmodulin-dependent protein phosphatase activity. Involved in several processes, including calcineurin-NFAT signaling cascade; peptidyl-serine dephosphorylation; and response to calcium ion. Located in several cellular components, including cytosol; dendritic spine; and nucleoplasm. Part of calcineurin complex. Colocalizes with cytoplasmic side of plasma membrane. Implicated in developmental and epileptic encephalopathy 91. Biomarker of focal segmental glomerulosclerosis and schizophrenia.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance