Reagents and instruments for immunology, cell biology and molecular biology.

Anti-PSENEN antibody (1-101) (Clone: S2MR)

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Cat# NB-22-48771-200

Size : 200uL(2x100uL)

Brand : Neo Biotech

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Western blot analysis of extracts of various cell lines, using PEN2/PSENEN rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Confocal imaging of paraffin-embedded Mouse brain using PEN2/PSENEN Rabbit monoclonal antibody followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
Immunofluorescence analysis of NIH-3T3 cells using PEN2/PSENEN rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Confocal imaging of NIH/3T3 cells using PEN2/PSENEN Rabbit monoclonal antibody followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Western blot analysis of various lysates using PEN2/PSENEN Rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

General Info

Host:	Rabbit
Applications:	WB/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-PEN2 (1-101) is suitable for use in Western Blot and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	S2MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	PSENEN
Gene ID:	55851
Uniprot ID:	PEN2_HUMAN
Immunogen Region:	1-101
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human PEN2/PSENEN (NP_758844.1)
Immunogen Sequence:	MNLERVSNEEKLNLCRKYYL GGFAFLPFLWLVNIFWFFRE AFLVPAYTEQSQIKGYVWRS AVGFLFWVIVLTSWITIFQI YRPRWGALGDYLSFTIPLGT P

Description

Tissue Specificity	Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.
Function	Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable). PSENEN modulates both endoproteolysis of presenilin and gamma-secretase activity.
Protein Name	Gamma-Secretase Subunit Pen-2 Presenilin Enhancer Protein 2
Database Links	Reactome: R-HSA-1251985 Reactome: R-HSA-193692 Reactome: R-HSA-205043 Reactome: R-HSA-2122948 Reactome: R-HSA-2644606 Reactome: R-HSA-2894862 Reactome: R-HSA-2979096 Reactome: R-HSA-3928665 Reactome: R-HSA-9013507 Reactome: R-HSA-9013700 Reactome: R-HSA-9017802 Reactome: R-HSA-977225
Cellular Localisation	Endoplasmic Reticulum Membrane Multi-Pass Membrane Protein Golgi Apparatus Golgi Stack Membrane Cell Membrane Membrane Predominantly Located In The Endoplasmic Reticulum And In The Cis-Golgi
Alternative Antibody Names	Anti-Gamma-Secretase Subunit Pen-2 antibody Anti-Presenilin Enhancer Protein 2 antibody Anti-PSENEN antibody Anti-PEN2 antibody Anti-MDS033 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance