Anti-Phospho-BTK antibody (544-561) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.6 µg/µl
Dilution Range:	WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	BTK
Gene ID:	695
Uniprot ID:	BTK_HUMAN
Immunogen Region:	544-561
Specificity:	Does not cross react to any other cellular protein.
Immunogen:	Synthetic peptide corresponding to Human BTK amino acids 544-561 (phopsho Y551). Sequence RYV LDD EY (p) T SSV GSK FPV

Tissue Specificity	Predominantly expressed in B-lymphocytes.
Post Translational Modifications	Following B-cell receptor (BCR) engagement, translocates to the plasma membrane where it gets phosphorylated at Tyr-551 by LYN and SYK. Phosphorylation at Tyr-551 is followed by autophosphorylation of Tyr-223 which may create a docking site for a SH2 containing protein. Phosphorylation at Ser-180 by PRKCB, leads in translocation of BTK back to the cytoplasmic fraction. Phosphorylation at Ser-21 and Ser-115 creates a binding site for PIN1 at these Ser-Pro motifs, and promotes it's recruitment.
Function	Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK also plays a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Acts as an activator of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.
Protein Name	Tyrosine-Protein Kinase Btk Agammaglobulinemia Tyrosine Kinase Atk B-Cell Progenitor Kinase Bpk Bruton Tyrosine Kinase
Database Links	Reactome: R-HSA-1236974 Reactome: R-HSA-166058 Reactome: R-HSA-2029482 Reactome: R-HSA-2424491 Reactome: R-HSA-2871809 Reactome: R-HSA-416476 Reactome: R-HSA-416482 Reactome: R-HSA-5602498 Reactome: R-HSA-5603041 Reactome: R-HSA-5663213 Reactome: R-HSA-8964315 Reactome: R-HSA-9664422 Reactome: R-HSA-9679191 Reactome: R-HSA-983695
Cellular Localisation	Cytoplasm Cell Membrane Peripheral Membrane Protein Nucleus Membrane Raft In Steady State Btk Is Predominantly Cytosolic Following B-Cell Receptor (Bcr) Engagement By Antigen Translocates To The Plasma Membrane Through Its Ph Domain Plasma Membrane Localization Is A Critical Step In The Activation Of Btk A Fraction Of Btk Also Shuttles Between The Nucleus And The Cytoplasm And Nuclear Export Is Mediated By The Nuclear Export Receptor Crm1
Alternative Antibody Names	Anti-Tyrosine-Protein Kinase Btk antibody Anti-Agammaglobulinemia Tyrosine Kinase antibody Anti-Atk antibody Anti-B-Cell Progenitor Kinase antibody Anti-Bpk antibody Anti-Bruton Tyrosine Kinase antibody Anti-BTK antibody Anti-AGMX1 antibody Anti-ATK antibody Anti-BPK antibody

Information sourced from Uniprot.org

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