XTT Cell Proliferation Assay Kit
Cat# CA031-S
Size : 500assays
Brand : Canvax Biotech
XTT Cell Proliferation Assay Kit
For an Optimized, Accurate & Sensitive Colorimetric Detection of Cellular Metabolic Activities
XTT Cell Proliferation Assay Kit is an optimized, accurate and sensitive colorimetric assay that detects the cellular metabolic activities. During the assay, the yellow tetrazolium salt XTT (sodium 2,3,-bis(2methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium) is reduced to a highly colored formazan dye by dehydrogenase enzymes in metabolically active cells.
This conversion only occurs in viable cells and thus, the amount of the formazan produced is proportional to viable cells in the sample. The formazan dye formed in the assay is soluble in aqueous solution and quantified by measuring the absorbance at wavelength 450 nm using a spectrophotometer. An electron coupling reagent, such as PMS (N-Methylphenazonium methyl sulphate), can significantly improve the efficiency of XTT reduction in cells.
Detailed information:
- Accurate: dye absorbance is proportional to the number of cells in each well.
- Sensitive: assayed even in low cell concentrations.
- Fast protocol: results within 2-5 hours with_tb_body et_pb_toggle_close">
Includes for 1,000 assays:
– 2 x 25 mL; 1 mL Activation Reagent
Datasheet MSDS- Spectrophotometric quantification of cell proliferation and viability in response to pharmaceutical, chemical, nutrients and environmental compounds.
- High Throughput Screening.
- Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
- Shipped at: _tb_body et_pb_toggle_close">
- Tavares Pereira, M., Kazemian, A., Rehrauer, H., & Kowalewski, M. P. (2022). Transcriptomic profiling of canine decidualization and effects of antigestagens on decidualized dog uterine stromal cells.(1), 21890.
- Kuczwara, V., Schuler, G., Pfarrer, C., Tiedje, L., Kazemian, A., Pereira, M. T., … & Klisch, K. (2023). Ultrastructural and Immunohistochemical Characterization of Maternal Myofibroblasts in the Bovine Placenta around Parturition.(1), 44.
- Hervás-Corpión, I., Navarro-Calvo, J., Martín-Climent, P., Iriarte-Gahete, M., Geribaldi-Doldán, N., Castro, C., & Valor, L. M. (2023). Defining a Correlative Transcriptional Signature Associated with Bulk Histone H3 Acetylation Levels in Adult Glioblastomas.(3), 374.
- Zaballos, M. A., Acuña-Ruiz, A., Morante, M., Riesco-Eizaguirre, G., Crespo, P., & Santisteban, P. (2022). Inhibiting ERK dimerization ameliorates BRAF-driven anaplastic thyroid cancer.(9), 1-19.
- Ramírez-Moya, J., Wert-Lamas, L., Acuña-Ruíz, A., Fletcher, A., Wert-Carvajal, C., McCabe, C. J., … & Riesco-Eizaguirre, G. (2022). Identification of an interactome network between lncRNAs and miRNAs in thyroid cancer reveals SPTY2D1-AS1 as a new tumor suppressor.(1), 1-13.
- Ramírez-Moya, Julia, et al. “An ADAR1-dependent RNA editing event in the cyclin-dependent kinase CDK13 promotes thyroid cancer hallmarks.” (2021): 1-20.
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