VEGF, Rat, Standard ABTS ELISA Development Kit

Cat# NB-50-1305-1000

Size : 1000Tests

Brand : Neo Biotech

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Product Specifications

Analytical sensitivity

Lot-specific

Assay range

Lot-specific

Hands-on time

1 hr 45 min

Time-to-result

24 hr 30 min

Homogenous (no wash)

No

Instrument

Absorbance Microplate Reader

Product size

10 x 96 Tests

Contents

This Rat VEGF-165 ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately.

Storage

Refer to product documentation for component specific storage temperature.

Protein name

VEGF-165

Protein family

Growth Factors & Receptors

Species (tested)

Rat

Assay kit format

ELISA Development Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

900-K436K consists of: 900-K436 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate).

Rat VEGF-165 ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant rat VEGF-165 in a sandwich ELISA format. Rat VEGF-165 ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells.

The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.

Target information

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.