Reagents and instruments for immunology, cell biology and molecular biology.

Ultra Sensitive Rat Insulin ELISA kit

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Cat# CC-90060

Size : 96tests

Brand : Crystal Chem

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Ultra Sensitive Rat Insulin ELISA kit

Product Specifications

Catalog #	90060
Sample Size	5 µL
Sample Types	Serum, plasma, cell culture, or fluid
Tests	96 wells (12 x 8 well strips)
Reagents	In liquid form (except standard)
Sensitivity	0.05 ng/mL
Assay Range	Low Range Assay: 0.1 - 6.4 ng/mL Wide Range Assay: 0.1 - 12.8 ng/mL High Range Assay: 1 - 64 ng/mL
Precision	Intra-assay precision CV ≤ 10.0% Inter-assay precision CV ≤ 10.0%
Storage	2-8°C
Regulatory	Research Use Only
Distribution	Worldwide
Assay Summary	Assay and protocol summary

Additional Info

Crystal Chem's is a worldwide leading supplier of mouse and rat insulin ELISA kits. Our kits are the most sensitive on the market, and are used by leading research universities, pharma companies, and government institutions around the world.

Note: For bulk purchases, save money with our 10-kit pack.

Highlights:

Small Sample Volume	Requires only a 5 µL sample, ideal for small quantity rat samples.
Highly Sensitive and Precise	This assay has a sensitivity of 0.05 ng/mL using a 5 µL sample with CV ≤ 10.0%.
Wide Dynamic Range	Range of 0.1 - 64.0 ng/mL, using only a 5 µL sample. Run both low and high insulin assays using the same kit.
Specific and Fast	This is an easy-to-use kit that is highly specific for rat insulin with results in under 3.5 hours.

Specificity:

Substance	Reactivity
Rat Insulin	100%
Mouse Insulin	100%*
Hamster Insulin	Detected*
Rat C-peptide	Not Detected
Rat pancreatic polypeptide	Not Detected
Porcine glucagon	Not Detected
Human Insulin like growth factor-I	Not Detected
Human Insulin like growth factor-II	Not Detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit

Typical Standard Curve:

References

Wojnacki, José, et al. “Tetraspanin-8 Sequesters Syntaxin-2 to Control Biphasic Release Propensity of Mucin Granules.” Nature Communications 2023; 14: 3710. Read more

Yang, Jiufang, et al. “Intestinal Farnesoid X Receptor Modulates Duodenal Surface Area but Does Not Control Glucose Absorption in Mice.” International Journal of Molecular Sciences 2023; 24: 4132. Read more

Frühbeck, Gema, et al. “Increased Aquaporin-7 Expression Is Associated with Changes in Rat Brown Adipose Tissue Whitening in Obesity: Impact of Cold Exposure and Bariatric Surgery.” International Journal of Molecular Sciences 2023; 24: 3412. Read more

Tasić, Danica, et al. “Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function.” International Journal of Molecular Sciences 2022; 23: 9023. Read more

Dahan, Tehila, et al. “Chronic Intermittent Hypoxia during Sleep Causes Browning of Interscapular Adipose Tissue Accompanied by Local Insulin Resistance in Mice.” International Journal of Molecular Sciences 2022; 23: 15462. Read more

de Castro Barbosa, Thais, et al. "High-fat diet reprograms the epigenome of rat spermatozoa and transgenerationally affects metabolism of the offspring." Molecular Metabolism 2016; 5: 184-197. Read more

Schlein, Christian, et al. "FGF21 lowers plasma triglycerides by accelerating lipoprotein catabolism in white and brown adipose tissues." Cell Metabolism 2016; 23: 441-453. Read more

Lipina, Christopher, et al. "NEU3 sialidase as a marker of insulin sensitivity: Regulation by fatty acids." Cellular Signalling 2015; 27: 1742-1750. Read more

Reynolds, Clare M., et al. "Maternal high‐fat diet‐induced programing of gut taste receptor and inflammatory gene expression in rat offspring is ameliorated by CLA supplementation." Physiological Reports 2015; 10: e12588. Read more

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