Trimethoprim [738-70-5]
Cat# HY-B0510-10g
Size : 10g
Brand : MedChemExpress
Trimethoprim is a bacteriostatic antibiotic and an orally active dihydrofolate reductase inhibitor. Trimethoprim is active against a wide range of Gram-positive and Gram-negative aerobic bacteria. Trimethoprim has the potential for the research of urinary tract infections, Shigellosis and Pneumocystis pneumonia. Trimethoprim can inhibit infection of Influenza A virus in chick embryo when combinated with zinc.
For research use only. We do not sell to patients.
Trimethoprim Chemical Structure
CAS No. : 738-70-5
This product is a controlled substance and not for sale in your territory.
Based on 6 publication(s) in Google Scholar
Other Forms of Trimethoprim:
- Trimethoprim lactate In-stock
- Trimethoprim-d9 In-stock
- Trimethoprim-d3 In-stock
- Trimethoprim sulfate Get quote
- Trimethoprim hydrochloride Get quote
- Trimethoprim-13C3 Get quote
View All Antibiotic Isoform Specific Products:
Description |
Trimethoprim is a bacteriostatic antibiotic and an orally active dihydrofolate reductase inhibitor. Trimethoprim is active against a wide range of Gram-positive and Gram-negative aerobic bacteria. Trimethoprim has the potential for the research of urinary tract infections, Shigellosis and Pneumocystis pneumonia. Trimethoprim can inhibit infection of Influenza A virus in chick embryo when combinated with zinc[1][2][3][4]. |
IC50 & Target |
Dihydrofolate reductase, Bacteria[1] |
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In Vitro |
Trimethoprim interrupts folate metabolism by inhibition of the activity of dihydrofolase reductase (DHFR), which reduces dihydrofolate to tetrahydrofolate (THF)[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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In Vivo |
Trimethoprim (10 mg/kg; i.v.; once every 12 h; 3 d) shows antibacterial activity against H. influenzae, S. pneumoniae, E. coli and N. meningitidis in infected mice[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Clinical Trial |
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Molecular Weight |
290.32 |
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Formula |
C14H18N4O3 |
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CAS No. |
738-70-5 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
NC1=NC=C(CC2=CC(OC)=C(OC)C(OC)=C2)C(N)=N1 |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
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Solvent & Solubility |
In Vitro:
DMSO : 50 mg/mL (172.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 0.67 mg/mL (2.31 mM; Need ultrasonic) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.98% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / DMSO | 1 mM | 3.4445 mL | 17.2224 mL | 34.4447 mL | 86.1119 mL |
DMSO | 5 mM | 0.6889 mL | 3.4445 mL | 6.8889 mL | 17.2224 mL |
10 mM | 0.3444 mL | 1.7222 mL | 3.4445 mL | 8.6112 mL | |
15 mM | 0.2296 mL | 1.1482 mL | 2.2963 mL | 5.7408 mL | |
20 mM | 0.1722 mL | 0.8611 mL | 1.7222 mL | 4.3056 mL | |
25 mM | 0.1378 mL | 0.6889 mL | 1.3778 mL | 3.4445 mL | |
30 mM | 0.1148 mL | 0.5741 mL | 1.1482 mL | 2.8704 mL | |
40 mM | 0.0861 mL | 0.4306 mL | 0.8611 mL | 2.1528 mL | |
50 mM | 0.0689 mL | 0.3444 mL | 0.6889 mL | 1.7222 mL | |
60 mM | 0.0574 mL | 0.2870 mL | 0.5741 mL | 1.4352 mL | |
80 mM | 0.0431 mL | 0.2153 mL | 0.4306 mL | 1.0764 mL | |
100 mM | 0.0344 mL | 0.1722 mL | 0.3444 mL | 0.8611 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Trimethoprim Related Classifications
- Infection
- Cell Cycle/DNA Damage Anti-infection
- Antifolate Bacterial Antibiotic Influenza Virus